Dec 2012



Advances in Free Flow Electrophorsis since 35 years

 Selected FFE Papers:

General FFE Publications

Free-flow electrophoresis for top-down proteomics by Fourier transform ion cyclotron resonance mass spectrometry.
Ouvry-Patat SA, Torres MP, Quek HH, Gelfand CA, O'Mullan P, Nissum M, Schroeder GK, Han J,
Elliott M, Dryhurst D, Ausio J, Wolfenden R, Borchers CH.
Proteomics. 2008 Jul;8(14):2798-808.

Free-flow electrophoresis in the proteomic era: a technique in flux.
Islinger M, Eckerskorn C, Völkl A.
Electrophoresis. 2010 Jun;31(11):1754-63. Review.

Top-down proteomics on a high-field Fourier transform ion cyclotron resonance mass spectrometer.
Ouvry-Patat SA, Torres MP, Gelfand CA, Quek HH, Easterling M, Speir JP, Borchers CH.
Methods Mol Biol. 2009;492:215-31.

Free-flow electrophoresis system for proteomics applications.
Weber G, Wildgruber R.
Methods Mol Biol. 2008;384:703-16.

Liquid-based free-flow electrophoresis-reversed-phase HPLC: a proteomic tool.
Moritz RL, Simpson RJ.
Nat Methods. 2005 Nov;2(11):863-73. No abstract available.

Recent developments in preparative free flow isoelectric focusing.
G. Weber and P. Bocek
Electrophoresis, 19, 1649 - 1653 (1998) 

Interval carrier free electrophoresis for high resolution protein purification. 
J. Bauer and G. Weber
J. Disp. Sci. Tech. 19 937-950 (1998)

Optimized continuous flow electrophoresis. 
G. Weber and P. Bocek
Electrophoresis, 17, 1906-1910 (1996)


Clinical Applications

Resolution of adiponectin oligomers in human plasma using free flow electrophoresis.
Foucher AL, Hartmann K, Hauptmann M, Wildgruber R, Safinowski M, Forst T, Pfützner A, Gelfand
CA, Nissum M.
Arch Physiol Biochem. 2009 Dec;115(5):267-78.

Analysis of human plasma proteins: a focus on sample collection and separation using free-flow electrophoresis.
Nissum M, Foucher AL.
Expert Rev Proteomics. 2008 Aug;5(4):571-87. Review.

Proteomic analysis of the secretome of human umbilical vein endothelial cells using a
combination of free-flow electrophoresis and nanoflow LC-MS/MS.
Tunica DG, Yin X, Sidibe A, Stegemann C, Nissum M, Zeng L, Brunet M, Mayr M.
Proteomics. 2009 Nov;9(21):4991-6.

Functional and complementary phosphorylation state attributes of human insulin-like growth
factor-binding protein-1 (IGFBP-1) isoforms resolved by free flow electrophoresis.
Nissum M, Abu Shehab M, Sukop U, Khosravi JM, Wildgruber R, Eckerskorn C, Han VK, Gupta MB.
Mol Cell Proteomics. 2009 Jun;8(6):1424-35. Epub 2009 Feb 3.

Proteomic analysis of colorectal cancer: prefractionation strategies using two-dimensional
free-flow electrophoresis.
Moritz RL, Skandarajah AR, Ji H, Simpson RJ.
Comp Funct Genomics. 2005;6(4):236-43.

Free-flow electrophoresis of the human urinary proteome.
Nissum M, Wildgruber R.
Methods Mol Biol. 2008;484:131-44.

Free-flow electrophoresis system for plasma proteomic applications.
Wildgruber R, Yi J, Nissum M, Eckerskorn C, Weber G.
Methods Mol Biol. 2008;424:287-300. Review.

Two-dimensional separation of human plasma proteins using iterative free-flow electrophoresis.Nissum M, Kuhfuss S, Hauptmann M, Obermaier C, Sukop U, Wildgruber R, Weber G, Eckerskorn C, Malmström J.
Proteomics. 2007 Dec;7(23):4218-27.

Breast cancer protein StarD10 identified by three-dimensional separation using free-flow
electrophoresis, reversed-phase high-performance liquid chromatography, and sodium dodecyl
sulfate-polyacrylamide gel electrophoresis.
Hoffmann P, Olayioye MA, Moritz RL, Lindeman GJ, Visvader JE, Simpson RJ, Kemp BE.
Electrophoresis. 2005 Mar;26(6):1029-37.

Continuous free-flow electrophoresis separation of cytosolic proteins from the human colon
carcinoma cell line LIM 1215: a non two-dimensional gel electrophoresis-based proteome analysis strategy.
Hoffmann P, Ji H, Moritz RL, Connolly LM, Frecklington DF, Layton MJ, Eddes JS, Simpson RJ.
Proteomics. 2001 Jul;1(7):807-18.

Application of free flow electrophoresis to the analysis of the urine proteome.
Foucher AL, Craft DR, Gelfand CA.
Methods Mol Biol. 2010;641:27-45.

Application of 2-D free-flow electrophoresis/RP-HPLC for proteomic analysis of human plasma
depleted of multi high-abundance proteins.
Moritz RL, Clippingdale AB, Kapp EA, Eddes JS, Ji H, Gilbert S, Connolly LM, Simpson RJ.
Proteomics. 2005 Aug;5(13):3402-13.

Efficient prefractionation of low-abundance proteins in human plasma and construction of a two-
dimensional map.
Cho SY, Lee EY, Lee JS, Kim HY, Park JM, Kwon MS, Park YK, Lee HJ, Kang MJ, Kim JY, Yoo JS, Park SJ, Cho JW, Kim HS, Paik YK.
Proteomics. 2005 Aug;5(13):3386-96.


Protein and Peptide Separations

Application of free-flow IEF to identify protein candidates changing under microgravity
Pietsch J, Kussian R, Sickmann A, Bauer J, Weber G, Nissum M, Westphal K, Egli M, Grosse J,
Schönberger J, Wildgruber R, Infanger M, Grimm D.
Proteomics. 2010 Mar;10(5):904-13. Erratum in: Proteomics. 2010 Apr;10(8):1728.

In-gel digestion of proteins using a solid-phase extraction microplate.
Nissum M, Schneider U, Kuhfuss S, Obermaier C, Wildgruber R, Posch A, Eckerskorn C.
Anal Chem. 2004 Apr 1;76(7):2040-5.

A combined free-flow electrophoresis and DIGE approach to identify proteins regulated by
butyrate in HT29 cells.
Fung KY, Cursaro C, Lewanowitsch T, Brierley GV, McColl SR, Lockett T, Head R, Hoffmann P,
Cosgrove L.
Proteomics. 2011 Mar;11(5):964-71. doi: 10.1002/pmic.201000429. Epub 2011 Jan 31.

Analysis of stage-specific expression of basic proteins in Leishmania infantum.
Brotherton MC, Racine G, Foucher AL, Drummelsmith J, Papadopoulou B, Ouellette M.
J Proteome Res. 2010 Aug 6;9(8):3842-53.

Enhancing mass spectrometry based serum profiling by a combination of free flow electrophoresis and ClinProt.
Hartwig S, Kotzka J, Müller H, Müller-Wieland D, Eckel J, Lehr S.
Arch Physiol Biochem. 2009 Dec;115(5):259-66.

Characterization and identification of proteases secreted by Aspergillus fumigatus using free
flow electrophoresis and MS.
Neustadt M, Costina V, Kupfahl C, Buchheidt D, Eckerskorn C, Neumaier M, Findeisen P.
Electrophoresis. 2009 Jun;30(12):2142-50.

Application of free-flow electrophoresis/2-dimentional gel electrophoresis for fractionation and
characterization of native proteome of Pseudomonas putida KT2440.
Choi CW, Hong YS, Kim SI.
J Microbiol. 2008 Aug;46(4):448-55. Epub 2008 Aug 31.

Free flow isoelectric focusing : a method for the separation of both hydrophilic and hydrophobic
proteins of rat liver peroxisomes.
Islinger M, Weber G.
Methods Mol Biol. 2008;432:199-215.

Optimized peptide separation and identification for mass spectrometry based proteomics via
free-flow electrophoresis.
Malmström J, Lee H, Nesvizhskii AI, Shteynberg D, Mohanty S, Brunner E, Ye M, Weber G,
Eckerskorn C, Aebersold R.
J Proteome Res. 2006 Sep;5(9):2241-9.

Free-flow isoelectric focusing of proteins remaining in cell fragments following sonication of
thyroid carcinoma cells.
Obermaier C, Jankowski V, Schmutzler C, Bauer J, Wildgruber R, Infanger M, Köhrle J, Krause E,
Weber G, Grimm D.
Electrophoresis. 2005 Jun;26(11):2109-16.


Membrane Proteins and Protein Complexes

Mammalian proteasome subpopulations with distinct molecular compositions and proteolytic
Drews O, Wildgruber R, Zong C, Sukop U, Nissum M, Weber G, Gomes AV, Ping P.
Mol Cell Proteomics. 2007 Nov;6(11):2021-31. Epub 2007 Jul 27.

Enhanced separation of membranes during free flow zonal electrophoresis in plants.
Barkla BJ, Vera-Estrella R, Pantoja O.
Anal Chem. 2007 Jul 15;79(14):5181-7. Epub 2007 Jun 13.

16-BAC/SDS-PAGE analysis of membrane proteins of yeast mitochondria purified by free flow
Braun RJ, Kinkl N, Zischka H, Ueffing M.
Methods Mol Biol. 2009;528:83-107.

Two-dimensional electrophoresis of membrane proteins.
Braun RJ, Kinkl N, Beer M, Ueffing M.
Anal Bioanal Chem. 2007 Oct;389(4):1033-45. Epub 2007 Aug 7. Review.


Organelle Separations

Improved proteome analysis of Saccharomyces cerevisiae mitochondria by free-flow
Zischka H, Weber G, Weber PJ, Posch A, Braun RJ, Bühringer D, Schneider U, Nissum M, Meitinger T, Ueffing M, Eckerskorn C.
Proteomics. 2003 Jun;3(6):906-16.

Peroxisomes from the heavy mitochondrial fraction: isolation by zonal free flow electrophoresis
and quantitative mass spectrometrical characterization.
Islinger M, Li KW, Loos M, Liebler S, Angermüller S, Eckerskorn C, Weber G, Abdolzade A, Völkl
J Proteome Res. 2010 Jan;9(1):113-24.

Novel proteins, putative membrane transporters, and an integrated metabolic network are revealed by quantitative proteomic analysis of Arabidopsis cell culture peroxisomes.
Eubel H, Meyer EH, Taylor NL, Bussell JD, O'Toole N, Heazlewood JL, Castleden I, Small ID, Smith SM, Millar AH.
Plant Physiol. 2008 Dec;148(4):1809-29. Epub 2008 Oct 17.

Electrophoretic analysis of the mitochondrial outer membrane rupture induced by permeability
Zischka H, Larochette N, Hoffmann F, Hamöller D, Jägemann N, Lichtmannegger J, Jennen L,
Müller-Höcker J, Roggel F, Göttlicher M, Vollmar AM, Kroemer G.Anal Chem. 2008 Jul 1;80

(13):5051-8. Epub 2008 May 30.

Purification of Saccharomyces cerevisiae mitochondria by zone electrophoresis in a free flow
Zischka H, Kinkl N, Braun RJ, Ueffing M.
Methods Mol Biol. 2008;432:51-64.

Isolation of highly pure rat liver mitochondria with the aid of zone-electrophoresis in a free
flow device (ZE-FFE).
Zischka H, Lichtmannegger J, Jägemann N, Jennen L, Hamöller D, Huber E, Walch A, Summer KH, Göttlicher M.
Methods Mol Biol. 2008;424:333-48. Review.

Free-flow electrophoresis for purification of plant mitochondria by surface charge.
Eubel H, Lee CP, Kuo J, Meyer EH, Taylor NL, Millar AH.
Plant J. 2007 Nov;52(3):583-94. Epub 2007 Aug 28.

Differential analysis of Saccharomyces cerevisiae mitochondria by free flow electrophoresis.
Zischka H, Braun RJ, Marantidis EP, Büringer D, Bornhövd C, Hauck SM, Demmer O, Gloeckner CJ, Reichert AS, Madeo F, Ueffing M.
Mol Cell Proteomics. 2006 Nov;5(11):2185-200. Epub 2006 Aug 17