Modified
Dec 2012
RW

 

FFE_01

Advances in Free Flow Electrophorsis since 35 years

 Schematic Overview of Free Flow Electrophoresis

FFE_scheme1

The sample is aspirated by a peristaltic pump and injected into a separation chamber consisting of two parallel plates. Under laminar flow, the sample is transported within a thin (0.4 - 0.1 mm) film of aqueous medium (proprietary ProLyte reagent).

The two plates are flanked by two electrodes which generate a high voltage electric field perpendicular to the laminar flow. Charged particles (proteins, peptides organelles,membrane fragments or whole cells) are deflected, allowing for subsequent separation and/or fractionation.
 

 

 Key features

Throughput. High sample recovery, exceptional reproducibility and rapid run times enable high throughput processing – within less than 30 minutes for separation & fractionation.

Reproducibility. Superior flexibility allows for collection of matrix-free fractions under native and denaturing conditions over both broad and narrow (0.1 pH or less) pH ranges.

Sensitivity. High resolution affords access to low-abundance proteins at very low concentrations.

Compatibility. Proprietary FFE technology ensures that fractionated samples are compatible with all downstream concentration procedures (e.g. ultrafiltration), separation techniques (e.g. two-dimensional electrophoresis or 2DE) and analytical methods (e.g. liquid chromatography/ mass spectrometry or LC/MS)

Reagents. Use of ProLytes, a patented, amphoteric reagent for Isoelectric Focusing (IEF), increases reproducibility due to the well defined mixture of small organic compounds.

 Applicational operating Modes

Preparative Isoelectric Focusing (IEF)

allows for high-resolution fractionation of proteins with recovery rates of up to 98%; separation of quantities up to 50 mg protein/h.
Five validated protocols are supplied with the instrument. 

 

 


 

FFE-IEF, isoelektric focussing mode

Zone-Electrophoresis

enables rapid separation of organelles, membranes and whole cells for the study of subproteomes.

 

 

 



 

FFE-ZE, zone electrophoresis mode

Iso-Tachophoresis

offers an enhanced range of separation methods based on electrophoretic mobility.











 

FFE-ITP, isotachophoresis mode

 Quality control

Separation of dye with different
isoelectric points (IEF mode).

Laminar flow in the separation chamber.